Vitronectin is a glycoprotein present in plasma and tissues. When circulating in the blood, Vitronectin is found as a mixture of 75kD and 65kD polypeptides. Vitronectin is used to promote cell attachment, adhesion, spreading, proliferation, migration and differentiation in a variety of normal and neoplastic cells. In addition to its cell spreading function, Vitronectin binds to heparin and collagen. GenDEPOT’s human Vitronectin is provided at a concentration of 0.5mg/ml with 0.1mg of Vitronectin being dissolved in 0.2ml of 0.15M NaCl, 0.005M HEPES buffer at approximately pH 7.4. Vitronectin is purified from human plasma by the method of Hayman et al(1) using an anti-Vitronectin monoclonal antibody affinity column and sterilized by 0.2um filtration.
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