TMB Stop Buffer (TSB) offers a unique combination of acid that produces a more stable stopped reaction product than other formulations of H2SO4 or HCl. Stopped reactions show increased absorbance values of approximately two-fold over unstopped reactions with less than 3% drift over six hours.
Store at RT
|Peak Wavelengths||650 nm, unstopped, blue reaction product
450 nm, stopped, yellow reaction product
|Reaction Temperature||Room temperature|
|Increased Absorbance Values||Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm.|
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