RPMI-1640 was developed in 1966 by Moore and his co-workers at Roswell Park Memorial Institute (hence the acronym RPMI). It is based on the RMPI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins.
While it was originally formulated to support lymphoblastoid is suspension culture, it has been proven to support a wide variety of anchorage-dependant cells. It has a wide range of uses, including the culture of fresh human lymphocytes, fusion protocols, and in the growth of hybrid cells.
- With L-Glutamine
- Without HEPES
|Phenol Red||5 mg/L|
Store at 2-8℃
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