Minimum Essential Medium (MEM) was developed by Harry Eagle and has become one of the most widely used of all synthetic cell culture media. The original formulation, containing Earle's Balanced Salts, was designed for use in propagating mouse L cells and HeLa cells. Eagle found that these cells require 13 amino acids and seven vitamins to grow and reproduce in vitro. MEM with Earle's Balanced Salts is suitable for cell culture in CO2 charged atmospheres, while MEM with Hank's Salts is suitable for cell culture in normal atmospheric conditions.
Store at 2-8℃
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