Affinity purified antibody isolated from a pool of
serum from goats immunizedwith purified
rat IgG was labeled with peroxidase by the
periodate method of Nakaneand Kawaoi
ELISA:1/1000 - 1/5000(1.0 ug/mL - 0.2 ug/mL)
Blotting:1/5000- 1/20000(0.2 ug/mL - 0.05 ug/mL)
Histo/Cytochemical Procedures:1/200 - 1/500
(5.0 ug/mL - 2.0 ug/mL)
Note: In many cases, the antibody may be diluted
further than indicated.
Store at 2-8℃
|Specificity/Cross Reactivity||Tested by gel diffusion and ELISA techniques as applicable. This product reacts specifically with rat IgG and may recognize other immunoglobulin types that have light chains in common with IgG. Reactivity to IgG subclasses has not been tested Antibodies to rat IgG may cross-react with immunoglobulins of other mammalian species if common binding sites are shared. Cross-reactivity with mouse serum has been minimized with affinity procedures.|
|Suggested Working Concentration||Optimal working concentration should be determined experimentally. Prepare working dilution in TBS or other buffer such as BSA or Milk Diluent/Blocking Solution.|
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