FastGreen Q-PCR Master Mix (2X), No ROX is a ready-to-use solution for performing accelerated quantitative real-time PCR on the BioRad: iCycler iQ, iQ5, MyiQTM, OpticonR, CFX 96, CFX 384, Roche: LightCycler®480, LightCycler®96, LightCycler®2.0,
iCycler iQ System, Corbett: Rotor-GeneTM 3000, 6000, Rotor-GeneQ, Eppendorf: MasterCycler TM ep realplex and Cepheid: Smart Cycler®.
The master mix includes Q-Fast Taq DNA polymerase that has been specifically engineered for faster replication, dNTPs, qGreen Fluorescent DNA binding Dye (substitute for SYBR Green I dye), ROX passive dye, and Mg2+ in an optimized PCR buffer. FastGreen Q-PCR Master contains unique heat-labile polymer that inactivate Taq DNA polymerase at room temperature and activate Taq DNA polymerase at 95oC in 10 sec. The unique mutant Taq and heat-labile polymer in combination with an optimized buffer ensures PCR specificity and sensitivity in accelerated processing time.
|Fast Taq Activation||FastGreen Q-PCR Master Mixes contains heat-labile polymer in combination with an optimized buffer ensures PCR specificity and sensitivity in accelerated processing time|
|Zero Primer Dimer in NTC||Q-Fast Taq DNA Polymerase and the optimized buffer eliminate non-specific amplification and formation of primer dimers.|
|Fast PCR Speed||Q-Fast Taq DNA Polymerase is the innovative hot-start DNA polymerase, newly engineered for rapid, robust performance.3x faster DNA synthesis and high inhibitor tolerance.|
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