Protein A is a cell wall component and four high affinity binding sites capable of binding specificaly to the Fc region of immunoglobin molecules from several species. The Protein A molecule is heat-stable and retains its native conformation, when exposed to denaturing reagents such as 4M urea, 4M thiocynate and 6M guanidine hydrochloride.
Puredown Protein A is covalently coupled to highly cross-linked agarose bead. This product is provided preblocked with fatty acid free bovine serum albumin and have been extensively washed to reduce-non-specific binding. Formulations are ready to use and 10ul of primary antibody can be used in standard immuno-precipitaion procedures.
|Reduced Background||Minus albumin-binding region to reduce non-specific binding|
|Convenient||Ready to use for immunoprecipitation procedures|
|Low Cost||Cost less than other competitor's Protein A/G-Agarose|
|Concentration||4% cross-linked agarose|
|Binding Capacity||> 35mg/ml Human IgG|
Store at 2-8℃
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